Journal: Aging (Albany NY)

Article Title: Tumor-derived exosomal circRNA_102481 contributes to EGFR-TKIs resistance via the miR-30a-5p/ROR1 axis in non-small cell lung cancer

doi: 10.18632/aging.203011

Figure Lengend Snippet: The RT-qPCR primers sequences.

Article Snippet: The exosomes RNA of five pair’s samples was extracted using RNeasy Mini Kit (Qiagen GmbH) and the circRNA profile was measured using Arraystar high-throughput human circRNA microarray V2.0 (8x15 K; Arraystar, Inc.).

Techniques:

Journal: Aging (Albany NY)

Article Title: Tumor-derived exosomal circRNA_102481 contributes to EGFR-TKIs resistance via the miR-30a-5p/ROR1 axis in non-small cell lung cancer

doi: 10.18632/aging.203011

Figure Lengend Snippet: circRNA_102481 is significantly up-regulated in serum exosomes of patients with EGFR-TKIs resistance. ( A ) Hierarchical clustering analysis showed the top 10 most increased and increased circRNAs in serum exosomes. ( B ) RT-qPCR validation.

Article Snippet: The exosomes RNA of five pair’s samples was extracted using RNeasy Mini Kit (Qiagen GmbH) and the circRNA profile was measured using Arraystar high-throughput human circRNA microarray V2.0 (8x15 K; Arraystar, Inc.).

Techniques: Quantitative RT-PCR, Biomarker Discovery

Journal: Aging (Albany NY)

Article Title: Tumor-derived exosomal circRNA_102481 contributes to EGFR-TKIs resistance via the miR-30a-5p/ROR1 axis in non-small cell lung cancer

doi: 10.18632/aging.203011

Figure Lengend Snippet: circRNA_102481 is mainly secreted via exosomes. ( A , B ) circRNA_102481 was unregulated in EGFR-TKIs resistant cells (PC9/GR and HCC827/ER), ** p < 0.01 versus matched sensitive cells (PC9 and HCC827). ( C , D ) circRNA_102481 was significantly up-regulated in exosomes of EGFR-TKIs resistant cells (PC9/GR and HCC827/ER), ** p < 0.01 versus matched sensitive cells (PC9 and HCC827).

Article Snippet: The exosomes RNA of five pair’s samples was extracted using RNeasy Mini Kit (Qiagen GmbH) and the circRNA profile was measured using Arraystar high-throughput human circRNA microarray V2.0 (8x15 K; Arraystar, Inc.).

Techniques:

Journal: Aging (Albany NY)

Article Title: Tumor-derived exosomal circRNA_102481 contributes to EGFR-TKIs resistance via the miR-30a-5p/ROR1 axis in non-small cell lung cancer

doi: 10.18632/aging.203011

Figure Lengend Snippet: Exosomal circRNA_102481 derived from EGFR-TKIs resistance cells. ( A ) circRNA_102481 expression levels in exosomes were almost equal to those in CCM, but, the levels of circRNA_102481 expression in EGFR-TKIs-resistant cells were significantly higher than in CCM and exosomes, # p > 0.05, * p < 0.05. ( B ) RT-qPCR was performed to detect the expression level of circRNA_102481 after treatment with 1 μg/ml RNase alone or combined with 0.1% Triton X-100 for 30 min, # p > 0.05, * p < 0.05 compared to the PBS control. ( C ) Transfection efficiency of circRNA_102481 was verified by a confocal laser scanning microscope and RT-qPCR assay. ** P < 0.01 versus si-NC group cells. ( D ) circRNA_102481 was significantly down- regulated in exosomes of si-circRNA_ 102481 cells. * p <0.05, ** p <0.01 versus si-NC group exosomes. CCM: cell culture medium.

Article Snippet: The exosomes RNA of five pair’s samples was extracted using RNeasy Mini Kit (Qiagen GmbH) and the circRNA profile was measured using Arraystar high-throughput human circRNA microarray V2.0 (8x15 K; Arraystar, Inc.).

Techniques: Derivative Assay, Expressing, Quantitative RT-PCR, Control, Transfection, Laser-Scanning Microscopy, Cell Culture

Journal: Aging (Albany NY)

Article Title: Tumor-derived exosomal circRNA_102481 contributes to EGFR-TKIs resistance via the miR-30a-5p/ROR1 axis in non-small cell lung cancer

doi: 10.18632/aging.203011

Figure Lengend Snippet: Functional validation assay of circRNA_102481 on EGFR-TKIs resistant cells in vitro (n=3). ( A , B ) MTT assay showed the effects of circRNA_102481 on EGFR-TKIs resistant cell proliferation. ( C , D ) Annexin V/PI assay showed the effects of circRNA_102481 on EGFR-TKIs resistant cell apoptosis. si-circRNA_102481: siRNA targeting circRNA_102481. ** p <0.01 versus si-NC group.

Article Snippet: The exosomes RNA of five pair’s samples was extracted using RNeasy Mini Kit (Qiagen GmbH) and the circRNA profile was measured using Arraystar high-throughput human circRNA microarray V2.0 (8x15 K; Arraystar, Inc.).

Techniques: Functional Assay, Biomarker Discovery, In Vitro, MTT Assay

Journal: Aging (Albany NY)

Article Title: Tumor-derived exosomal circRNA_102481 contributes to EGFR-TKIs resistance via the miR-30a-5p/ROR1 axis in non-small cell lung cancer

doi: 10.18632/aging.203011

Figure Lengend Snippet: Functional validation assay of exosomes circRNA_102481 on EGFR-TKIs resistant cells in vitro (n=3). ( A , B ) MTT assay showed the effects of exosomes circRNA_102481 on EGFR-TKIs resistant cell proliferation. ( C , D ) Annexin V/PI assay showed the effects of exosomes circRNA_102481 on EGFR-TKIs resistant cell apoptosis. si-circRNA_102481: siRNA targeting circRNA_102481. * p <0.05 versus si-NC group.

Article Snippet: The exosomes RNA of five pair’s samples was extracted using RNeasy Mini Kit (Qiagen GmbH) and the circRNA profile was measured using Arraystar high-throughput human circRNA microarray V2.0 (8x15 K; Arraystar, Inc.).

Techniques: Functional Assay, Biomarker Discovery, In Vitro, MTT Assay

Journal: Aging (Albany NY)

Article Title: Tumor-derived exosomal circRNA_102481 contributes to EGFR-TKIs resistance via the miR-30a-5p/ROR1 axis in non-small cell lung cancer

doi: 10.18632/aging.203011

Figure Lengend Snippet: Functional validation assay of exosomes circRNA_102481 on EGFR-TKIs sensitive cells in vitro (n=3). ( A , B ) MTT assay showed the effects of circRNA_102481 on EGFR-TKIs sensitive cell proliferation. ( C , D ) Annexin V/PI assay showed the effects of circRNA_102481 on EGFR-TKIs sensitive cell apoptosis. circRNA_100859 vector: circRNA_100859 overexpression vector. * p <0.05 versus blank vector group.

Article Snippet: The exosomes RNA of five pair’s samples was extracted using RNeasy Mini Kit (Qiagen GmbH) and the circRNA profile was measured using Arraystar high-throughput human circRNA microarray V2.0 (8x15 K; Arraystar, Inc.).

Techniques: Functional Assay, Biomarker Discovery, In Vitro, MTT Assay, Plasmid Preparation, Over Expression

Journal: Aging (Albany NY)

Article Title: Tumor-derived exosomal circRNA_102481 contributes to EGFR-TKIs resistance via the miR-30a-5p/ROR1 axis in non-small cell lung cancer

doi: 10.18632/aging.203011

Figure Lengend Snippet: circRNA_102481 serves as a miR-30a-5p sponge to regulate ROR1 expression. The detailed potential binding sequences of circRNA_102481 to miR-30a-5p ( A ) and ROR1 to miR-30a-5p ( D ). miR-30a-5p of PC9/GR and HCC827/ER cells was abundantly pulled down by circRNA_102481 ( B ) or ROR1 ( E ). Dual-luciferase reporter assay confirmed that miR-30a-5p competitively targeted circRNA_102481( C ), and that ROR1 is a direct target of miR-30a-5p ( F ). # p > 0.05, * p < 0.05, ** p <0.01.

Article Snippet: The exosomes RNA of five pair’s samples was extracted using RNeasy Mini Kit (Qiagen GmbH) and the circRNA profile was measured using Arraystar high-throughput human circRNA microarray V2.0 (8x15 K; Arraystar, Inc.).

Techniques: Expressing, Binding Assay, Luciferase, Reporter Assay

Journal: Aging (Albany NY)

Article Title: Tumor-derived exosomal circRNA_102481 contributes to EGFR-TKIs resistance via the miR-30a-5p/ROR1 axis in non-small cell lung cancer

doi: 10.18632/aging.203011

Figure Lengend Snippet: Effects of exosomes on circRNA_102481-miR-30a-5p-ROR1 Axis. ( A , B ) RT-qPCR assay showed miR-30a-5p expression in PC9/GR or HCC827/ER cells of si-NC and si-circRNA_102481 groups. ( C ) Transfection efficiency of miR-30a-5p mimic and miR-30a-5p inhibitor was verified by RT-qPCR assay. ** p <0.01 versus miR-30a-5p NC group cells. ( D , E ) qRT-PCR demonstrated that ROR1 mRNA expression were significantly decreased in miR-30a-5p mimic group, and increased in miR-30a-5p inhibitor group. * p <0.05, ** p <0.01 versus miR-30a-5p NC group.

Article Snippet: The exosomes RNA of five pair’s samples was extracted using RNeasy Mini Kit (Qiagen GmbH) and the circRNA profile was measured using Arraystar high-throughput human circRNA microarray V2.0 (8x15 K; Arraystar, Inc.).

Techniques: Quantitative RT-PCR, Expressing, Transfection

Journal: Aging (Albany NY)

Article Title: Tumor-derived exosomal circRNA_102481 contributes to EGFR-TKIs resistance via the miR-30a-5p/ROR1 axis in non-small cell lung cancer

doi: 10.18632/aging.203011

Figure Lengend Snippet: Effects of exosomes on circRNA_102481-miR-30a-5p-ROR1 Axis. ( A , B ) Relative ROR1 mRNA and protein were analyzed in PC9/GR and HCC827/ER cells the cells were co-cultured with exosomes of the si-NC, si-circRNA_102481, and miR-30a-5p NC, miR-30a-5p inhibitor using RT-qPCR and western blot assay, respectively. ( C ) IC50 of gefitinib or erlotinib the cells were co-cultured with exosomes of the si-NC, si-circRNA_102481, and miR-30a-5p NC, miR-30a-5p inhibitor was detected by MTT assay. * p < 0.05, ** p <0.01.

Article Snippet: The exosomes RNA of five pair’s samples was extracted using RNeasy Mini Kit (Qiagen GmbH) and the circRNA profile was measured using Arraystar high-throughput human circRNA microarray V2.0 (8x15 K; Arraystar, Inc.).

Techniques: Cell Culture, Quantitative RT-PCR, Western Blot, MTT Assay

Journal: Aging (Albany NY)

Article Title: Tumor-derived exosomal circRNA_102481 contributes to EGFR-TKIs resistance via the miR-30a-5p/ROR1 axis in non-small cell lung cancer

doi: 10.18632/aging.203011

Figure Lengend Snippet: Large sample validation and correlation analysis. ( A – C ) RT-qPCR analysis of exosomes circRNA_102481, exosomes miR-30a-5p, exosomes ROR1 mRNA before and after EGFR-TKIs resistance in 58 NSCLC patients. ( D , E ) Pearson’s correlation analysis of between exosomes circRNA_102481 and exosomes miR-30a-5p, between exosomes miR-30a-5p and exosomes ROR1 mRNA.

Article Snippet: The exosomes RNA of five pair’s samples was extracted using RNeasy Mini Kit (Qiagen GmbH) and the circRNA profile was measured using Arraystar high-throughput human circRNA microarray V2.0 (8x15 K; Arraystar, Inc.).

Techniques: Biomarker Discovery, Quantitative RT-PCR

Journal: Aging (Albany NY)

Article Title: Tumor-derived exosomal circRNA_102481 contributes to EGFR-TKIs resistance via the miR-30a-5p/ROR1 axis in non-small cell lung cancer

doi: 10.18632/aging.203011

Figure Lengend Snippet: Relationship between exosomes circRNA_102481-miR-30a-5p-ROR1 axis with clinicopathological variables. ( A ) exosomes circRNA_102481, ( B ) exosomes miR-30a-5p, ( C ) exosomes ROR1 mRNA.

Article Snippet: The exosomes RNA of five pair’s samples was extracted using RNeasy Mini Kit (Qiagen GmbH) and the circRNA profile was measured using Arraystar high-throughput human circRNA microarray V2.0 (8x15 K; Arraystar, Inc.).

Techniques:

Journal: Aging (Albany NY)

Article Title: Tumor-derived exosomal circRNA_102481 contributes to EGFR-TKIs resistance via the miR-30a-5p/ROR1 axis in non-small cell lung cancer

doi: 10.18632/aging.203011

Figure Lengend Snippet: Kaplan-Meier PFS curve stratified by exosomes circRNA_102481- miR-30a-5p-ROR1 axis expression. PFS duration between higher and lower ( A ) exosomes circRNA_102481, ( B ) exosomes miR-30a-5p, ( C ) exosomes ROR1 mRNA.

Article Snippet: The exosomes RNA of five pair’s samples was extracted using RNeasy Mini Kit (Qiagen GmbH) and the circRNA profile was measured using Arraystar high-throughput human circRNA microarray V2.0 (8x15 K; Arraystar, Inc.).

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: Tumor-derived exosomal circRNA_102481 contributes to EGFR-TKIs resistance via the miR-30a-5p/ROR1 axis in non-small cell lung cancer

doi: 10.18632/aging.203011

Figure Lengend Snippet: Kaplan-Meier OS curve stratified by exosomes circRNA_102481- miR-30a-5p-ROR1 axis expression. OS duration between higher and lower ( A ) exosomes circRNA_102481, ( B ) exosomes miR-30a-5p, ( C ) exosomes ROR1 mRNA.

Article Snippet: The exosomes RNA of five pair’s samples was extracted using RNeasy Mini Kit (Qiagen GmbH) and the circRNA profile was measured using Arraystar high-throughput human circRNA microarray V2.0 (8x15 K; Arraystar, Inc.).

Techniques: Expressing

Journal: Signal Transduction and Targeted Therapy

Article Title: CircRNA DICAR as a novel endogenous regulator for diabetic cardiomyopathy and diabetic pyroptosis of cardiomyocytes

doi: 10.1038/s41392-022-01306-2

Figure Lengend Snippet: Identified circRNA expression in diabetic cardiomyopathy mouse. a Microarray identified in the hearts of db/db mice. b Select significantly regulated circRNA detected by qPCR ( n = 9). c CircRNA resistance detected by RNAse. d Mm9-circ-008009 structure. e Identification of the mm9-circ-008009 back-spliced junction sites. f Identification of the hsa-circ-0131202 back-spliced junction sites. g , h Sanger sequence of mm9-circ-008009 and hsa-circ-0131202. i , j Second structure prediction of mm9-circ-008009 and hsa-circ-0131202. k FISH detection of mm9-circ-008009 in the mouse heart tissues. Data are represented as mean ± SEM. ** P < 0.01 vs . WT group

Article Snippet: The labeled cRNAs were hybridized onto the Arraystar Mouse circRNA Array (8 x 15 K, Arraystar).

Techniques: Expressing, Microarray, Sequencing

Journal: Genomics Data

Article Title: Noncoding RNAs in human intervertebral disc degeneration: An integrated microarray study

doi: 10.1016/j.gdata.2015.05.027

Figure Lengend Snippet:

Article Snippet: Sequencer or array type , miRNA microarray: miRCURYTM LNA Array (v.18.0, Exiqon) lncRNA microarray: Arraystar Human LncRNA Array (v2.0, Arraystar) circRNA microarray: Arraystar Human circRNA Array (8x15K, Arraystar).

Techniques: Microarray, Microscopy